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ProteoSep HPRP column - is a unique NPS [non-porous silica] based HPLC column designed specifically for Proteins and Peptides. These analytes are usually found in very complex samples and in many cases are present with a numer of detergents, chaotropes and additives typical to protein and peptide solubilization. As such, special bonding chemistries are required to make the C-18 hydrophobic bonding extremely stable to these additives asw well as accomodate the complex nature of proteins themselves. These surfaces are also required to withstand high pH [>9.5] samples and still retain longevity and resolving power. The HPRP column technology provides for this stability as well as the non porous nature of the support eliminating the need for extensive regeneration protocols required for all other silica based supports.
The HPRP column design works with any HPLC vendor and now brings the separation and resolving power of HPLC that has served the small molecule analysis community for decades to routine protein and peptide analysis laboratories.
Eprogen aslo has other NPS columns specially bonded for small molecule separations - see below:
Micra NPS® C-18 HPLC Technical Note
Short Run Times - Reduced MP Solvent Usage
High Sensitivity & Resolution
Greatly Improved Mass Transfer Kinetics
Increased pH stability
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ODS-I
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MICRA NPS ODS-I - polymeric C-18 bonded phase on 1.5µ
NPS.
See Application Note
See Application Note
See Application Note
See Application Note
Small drug molecules to biomolecules can be rapidly analyzed with ODS-I columns. With the addition of modifiers such as TEA, excellent peak shapes for many basic compounds such as TCA's can be obtained. This is an excellent column for high throughput and QC lab applications as well as LC/MS separations. The absence of porosity in NPS columns makes them suitable for the direct injection of plasma for metabolite analysis.
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ODS-IIIE
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MICRA NPS ODS-IIIE - Endcapped C-18 bonded phase on 1.5µ
NPS
See Application Note
This is an excellent column for high throughput and QC lab applications, as well as LC/MS separations. The endcapping on ODS-IIIE columns delivers excellent resolution and analysis speed with low peak tailing for basic drug compounds.
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MICRA NPS TAS - a C-30 coating on 1.5µ NPS for use in specialty separations
See application Note
See Application Note
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NPS-Sil
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MICRA NPS-Sil - non-bonded 1.5µ
NPS.
These columns are highly efficient for all normal phase applications and work particularly well with very polar compounds. The surface silanols are only slightly acidic.
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NPS-PAH
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MICRA NPS-PAH - polymeric bonded C18 phase on 1.5µ NPS.
See Application Note
This PAH column offer high sensitivity, superior reproducibility, and the baseline separation of 16 PAH's in less than 10 minutes.
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